Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_6_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 46455148
Single-end alignments with a unique best hit 2013278
Sequences without alignments under any condition 42132594
Sequences that did not map uniquely 2309276
Genomic sequence context not extractable (edges of chromosomes) 69

Cytosine Methylation

Total C's analysed 12294135
Methylated C's in CpG context 1280460
Methylated C's in CHG context 56701
Methylated C's in CHH context 642287
Methylated C's in Unknown context 42
Unmethylated C's in CpG context 536403
Unmethylated C's in CHG context 2079296
Unmethylated C's in CHH context 7698988
Unmethylated C's in Unknown context 457
Percentage methylation (CpG context) 70.5%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 7.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 403589 original top strand
CTOT 576906 complementary to original top strand
CTOB 596358 complementary to original bottom strand
OB 436356 original bottom strand

Deduplication

Alignments analysed 2013209
Unique alignments 384179
Duplicates removed 1629030
Duplicated alignments were found at 170292 different positions

Cytosine Methylation after Extraction

Total C's analysed 2354787
Methylated C's in CpG context 198359
Methylated C's in CHG context 12844
Methylated C's in CHH context 216864
Unmethylated C's in CpG context 101931
Unmethylated C's in CHG context 320460
Unmethylated C's in CHH context 1504329
Percentage methylation (CpG context) 66.1%
Percentage methylation (CHG context) 3.9%
Percentage methylation (CHH context) 12.6%

M-Bias Plot