Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 2079533
Sequences without alignments under any condition 42966736
Sequences that did not map uniquely 2355467
Genomic sequence context not extractable (edges of chromosomes) 73

Cytosine Methylation

Total C's analysed 12804963
Methylated C's in CpG context 1404189
Methylated C's in CHG context 61962
Methylated C's in CHH context 717589
Methylated C's in Unknown context 50
Unmethylated C's in CpG context 670203
Unmethylated C's in CHG context 2249103
Unmethylated C's in CHH context 7701917
Unmethylated C's in Unknown context 461
Percentage methylation (CpG context) 67.7%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 8.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 426898 original top strand
CTOT 580972 complementary to original top strand
CTOB 608521 complementary to original bottom strand
OB 463069 original bottom strand

Deduplication

Alignments analysed 2079460
Unique alignments 388651
Duplicates removed 1690809
Duplicated alignments were found at 171673 different positions

Cytosine Methylation after Extraction

Total C's analysed 2397810
Methylated C's in CpG context 196351
Methylated C's in CHG context 13520
Methylated C's in CHH context 238368
Unmethylated C's in CpG context 123953
Unmethylated C's in CHG context 328465
Unmethylated C's in CHH context 1497153
Percentage methylation (CpG context) 61.3%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 13.7%

M-Bias Plot