Alignment Stats
| Sequences analysed in total |
44905078 |
| Single-end alignments with a unique best hit |
2010139 |
| Sequences without alignments under any condition |
40784777 |
| Sequences that did not map uniquely |
2110162 |
| Genomic sequence context not extractable (edges of chromosomes) |
74 |
|
|
Cytosine Methylation
| Total C's analysed |
12404965 |
| Methylated C's in CpG context |
1806777 |
| Methylated C's in CHG context |
69275 |
| Methylated C's in CHH context |
620239 |
| Methylated C's in Unknown context |
50 |
| Unmethylated C's in CpG context |
510473 |
| Unmethylated C's in CHG context |
2191210 |
| Unmethylated C's in CHH context |
7206991 |
| Unmethylated C's in Unknown context |
290 |
| Percentage methylation (CpG context) |
78.0% |
| Percentage methylation (CHG context) |
3.1% |
| Percentage methylation (CHH context) |
7.9% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
407301 |
original top strand |
| CTOT |
568615 |
complementary to original top strand |
| CTOB |
594371 |
complementary to original bottom strand |
| OB |
439778 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
2010065 |
| Unique alignments |
339620 |
| Duplicates removed |
1670445 |
| Duplicated alignments were found at 156416 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2113978 |
| Methylated C's in CpG context |
222919 |
| Methylated C's in CHG context |
13165 |
| Methylated C's in CHH context |
199090 |
| Unmethylated C's in CpG context |
81651 |
| Unmethylated C's in CHG context |
288530 |
| Unmethylated C's in CHH context |
1308623 |
| Percentage methylation (CpG context) |
73.2% |
| Percentage methylation (CHG context) |
4.4% |
| Percentage methylation (CHH context) |
13.2% |
|
|
M-Bias Plot