Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 44905078
Single-end alignments with a unique best hit 2010139
Sequences without alignments under any condition 40784777
Sequences that did not map uniquely 2110162
Genomic sequence context not extractable (edges of chromosomes) 74

Cytosine Methylation

Total C's analysed 12404965
Methylated C's in CpG context 1806777
Methylated C's in CHG context 69275
Methylated C's in CHH context 620239
Methylated C's in Unknown context 50
Unmethylated C's in CpG context 510473
Unmethylated C's in CHG context 2191210
Unmethylated C's in CHH context 7206991
Unmethylated C's in Unknown context 290
Percentage methylation (CpG context) 78.0%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 7.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 407301 original top strand
CTOT 568615 complementary to original top strand
CTOB 594371 complementary to original bottom strand
OB 439778 original bottom strand

Deduplication

Alignments analysed 2010065
Unique alignments 339620
Duplicates removed 1670445
Duplicated alignments were found at 156416 different positions

Cytosine Methylation after Extraction

Total C's analysed 2113978
Methylated C's in CpG context 222919
Methylated C's in CHG context 13165
Methylated C's in CHH context 199090
Unmethylated C's in CpG context 81651
Unmethylated C's in CHG context 288530
Unmethylated C's in CHH context 1308623
Percentage methylation (CpG context) 73.2%
Percentage methylation (CHG context) 4.4%
Percentage methylation (CHH context) 13.2%

M-Bias Plot