Alignment Stats
| Sequences analysed in total |
48376729 |
| Single-end alignments with a unique best hit |
2160248 |
| Sequences without alignments under any condition |
43980854 |
| Sequences that did not map uniquely |
2235627 |
| Genomic sequence context not extractable (edges of chromosomes) |
70 |
|
|
Cytosine Methylation
| Total C's analysed |
13230157 |
| Methylated C's in CpG context |
1864732 |
| Methylated C's in CHG context |
69400 |
| Methylated C's in CHH context |
642023 |
| Methylated C's in Unknown context |
54 |
| Unmethylated C's in CpG context |
595943 |
| Unmethylated C's in CHG context |
2273419 |
| Unmethylated C's in CHH context |
7784640 |
| Unmethylated C's in Unknown context |
344 |
| Percentage methylation (CpG context) |
75.8% |
| Percentage methylation (CHG context) |
3.0% |
| Percentage methylation (CHH context) |
7.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
439520 |
original top strand |
| CTOT |
602057 |
complementary to original top strand |
| CTOB |
641023 |
complementary to original bottom strand |
| OB |
477578 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
2160178 |
| Unique alignments |
364420 |
| Duplicates removed |
1795758 |
| Duplicated alignments were found at 168982 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2247377 |
| Methylated C's in CpG context |
220270 |
| Methylated C's in CHG context |
13219 |
| Methylated C's in CHH context |
205636 |
| Unmethylated C's in CpG context |
97022 |
| Unmethylated C's in CHG context |
300651 |
| Unmethylated C's in CHH context |
1410579 |
| Percentage methylation (CpG context) |
69.4% |
| Percentage methylation (CHG context) |
4.2% |
| Percentage methylation (CHH context) |
12.7% |
|
|
M-Bias Plot