Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_3_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 48376729
Single-end alignments with a unique best hit 2160248
Sequences without alignments under any condition 43980854
Sequences that did not map uniquely 2235627
Genomic sequence context not extractable (edges of chromosomes) 70

Cytosine Methylation

Total C's analysed 13230157
Methylated C's in CpG context 1864732
Methylated C's in CHG context 69400
Methylated C's in CHH context 642023
Methylated C's in Unknown context 54
Unmethylated C's in CpG context 595943
Unmethylated C's in CHG context 2273419
Unmethylated C's in CHH context 7784640
Unmethylated C's in Unknown context 344
Percentage methylation (CpG context) 75.8%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 7.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 439520 original top strand
CTOT 602057 complementary to original top strand
CTOB 641023 complementary to original bottom strand
OB 477578 original bottom strand

Deduplication

Alignments analysed 2160178
Unique alignments 364420
Duplicates removed 1795758
Duplicated alignments were found at 168982 different positions

Cytosine Methylation after Extraction

Total C's analysed 2247377
Methylated C's in CpG context 220270
Methylated C's in CHG context 13219
Methylated C's in CHH context 205636
Unmethylated C's in CpG context 97022
Unmethylated C's in CHG context 300651
Unmethylated C's in CHH context 1410579
Percentage methylation (CpG context) 69.4%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 12.7%

M-Bias Plot