Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/v082/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 46105140 Number of alignments with a unique best hit from the different alignments: 2050964 Mapping efficiency: 4.4% Sequences with no alignments under any condition: 41863391 Sequences did not map uniquely: 2190785 Sequences which were discarded because genomic sequence could not be extracted: 63 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 413480 ((converted) top strand) CT/GA: 445517 ((converted) bottom strand) GA/CT: 579519 (complementary to (converted) top strand) GA/GA: 612385 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 12647847 Total methylated C's in CpG context: 1678932 Total methylated C's in CHG context: 69149 Total methylated C's in CHH context: 679879 Total methylated C's in Unknown context: 82 Total unmethylated C's in CpG context: 579763 Total unmethylated C's in CHG context: 2266089 Total unmethylated C's in CHH context: 7374035 Total unmethylated C's in Unknown context: 359 C methylated in CpG context: 74.3% C methylated in CHG context: 3.0% C methylated in CHH context: 8.4% C methylated in Unknown context (CN or CHN): 18.6% Bismark completed in 0d 0h 23m 40s