Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 46105140
Single-end alignments with a unique best hit 2050964
Sequences without alignments under any condition 41863391
Sequences that did not map uniquely 2190785
Genomic sequence context not extractable (edges of chromosomes) 63

Cytosine Methylation

Total C's analysed 12647847
Methylated C's in CpG context 1678932
Methylated C's in CHG context 69149
Methylated C's in CHH context 679879
Methylated C's in Unknown context 82
Unmethylated C's in CpG context 579763
Unmethylated C's in CHG context 2266089
Unmethylated C's in CHH context 7374035
Unmethylated C's in Unknown context 359
Percentage methylation (CpG context) 74.3%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 8.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 413480 original top strand
CTOT 579519 complementary to original top strand
CTOB 612385 complementary to original bottom strand
OB 445517 original bottom strand

Deduplication

Alignments analysed 2050901
Unique alignments 351822
Duplicates removed 1699079
Duplicated alignments were found at 162203 different positions

Cytosine Methylation after Extraction

Total C's analysed 2184474
Methylated C's in CpG context 213059
Methylated C's in CHG context 13257
Methylated C's in CHH context 216278
Unmethylated C's in CpG context 94747
Unmethylated C's in CHG context 300551
Unmethylated C's in CHH context 1346582
Percentage methylation (CpG context) 69.2%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 13.8%

M-Bias Plot