Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_1_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 46792286
Single-end alignments with a unique best hit 2117445
Sequences without alignments under any condition 42430582
Sequences that did not map uniquely 2244259
Genomic sequence context not extractable (edges of chromosomes) 52

Cytosine Methylation

Total C's analysed 13077617
Methylated C's in CpG context 1860212
Methylated C's in CHG context 70565
Methylated C's in CHH context 647883
Methylated C's in Unknown context 68
Unmethylated C's in CpG context 609554
Unmethylated C's in CHG context 2297560
Unmethylated C's in CHH context 7591843
Unmethylated C's in Unknown context 342
Percentage methylation (CpG context) 75.3%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 7.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 432543 original top strand
CTOT 585370 complementary to original top strand
CTOB 625468 complementary to original bottom strand
OB 474012 original bottom strand

Deduplication

Alignments analysed 2117393
Unique alignments 359138
Duplicates removed 1758255
Duplicated alignments were found at 165505 different positions

Cytosine Methylation after Extraction

Total C's analysed 2224066
Methylated C's in CpG context 219081
Methylated C's in CHG context 13541
Methylated C's in CHH context 210878
Unmethylated C's in CpG context 100089
Unmethylated C's in CHG context 301405
Unmethylated C's in CHH context 1379072
Percentage methylation (CpG context) 68.6%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 13.3%

M-Bias Plot