Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/v082/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 55048106 Number of alignments with a unique best hit from the different alignments: 2536705 Mapping efficiency: 4.6% Sequences with no alignments under any condition: 49739989 Sequences did not map uniquely: 2771412 Sequences which were discarded because genomic sequence could not be extracted: 75 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 501017 ((converted) top strand) CT/GA: 539128 ((converted) bottom strand) GA/CT: 728586 (complementary to (converted) top strand) GA/GA: 767899 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 15638469 Total methylated C's in CpG context: 2023305 Total methylated C's in CHG context: 79670 Total methylated C's in CHH context: 767014 Total methylated C's in Unknown context: 64 Total unmethylated C's in CpG context: 710544 Total unmethylated C's in CHG context: 2845824 Total unmethylated C's in CHH context: 9212112 Total unmethylated C's in Unknown context: 425 C methylated in CpG context: 74.0% C methylated in CHG context: 2.7% C methylated in CHH context: 7.7% C methylated in Unknown context (CN or CHN): 13.1% Bismark completed in 0d 0h 28m 25s