Alignment Stats
| Sequences analysed in total |
55048106 |
| Single-end alignments with a unique best hit |
2536705 |
| Sequences without alignments under any condition |
49739989 |
| Sequences that did not map uniquely |
2771412 |
| Genomic sequence context not extractable (edges of chromosomes) |
75 |
|
|
Cytosine Methylation
| Total C's analysed |
15638469 |
| Methylated C's in CpG context |
2023305 |
| Methylated C's in CHG context |
79670 |
| Methylated C's in CHH context |
767014 |
| Methylated C's in Unknown context |
64 |
| Unmethylated C's in CpG context |
710544 |
| Unmethylated C's in CHG context |
2845824 |
| Unmethylated C's in CHH context |
9212112 |
| Unmethylated C's in Unknown context |
425 |
| Percentage methylation (CpG context) |
74.0% |
| Percentage methylation (CHG context) |
2.7% |
| Percentage methylation (CHH context) |
7.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
501017 |
original top strand |
| CTOT |
728586 |
complementary to original top strand |
| CTOB |
767899 |
complementary to original bottom strand |
| OB |
539128 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
2536630 |
| Unique alignments |
384697 |
| Duplicates removed |
2151933 |
| Duplicated alignments were found at 182700 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2391747 |
| Methylated C's in CpG context |
228558 |
| Methylated C's in CHG context |
13938 |
| Methylated C's in CHH context |
225068 |
| Unmethylated C's in CpG context |
102063 |
| Unmethylated C's in CHG context |
328800 |
| Unmethylated C's in CHH context |
1493320 |
| Percentage methylation (CpG context) |
69.1% |
| Percentage methylation (CHG context) |
4.1% |
| Percentage methylation (CHH context) |
13.1% |
|
|
M-Bias Plot