Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_18_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 55048106
Single-end alignments with a unique best hit 2536705
Sequences without alignments under any condition 49739989
Sequences that did not map uniquely 2771412
Genomic sequence context not extractable (edges of chromosomes) 75

Cytosine Methylation

Total C's analysed 15638469
Methylated C's in CpG context 2023305
Methylated C's in CHG context 79670
Methylated C's in CHH context 767014
Methylated C's in Unknown context 64
Unmethylated C's in CpG context 710544
Unmethylated C's in CHG context 2845824
Unmethylated C's in CHH context 9212112
Unmethylated C's in Unknown context 425
Percentage methylation (CpG context) 74.0%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 7.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 501017 original top strand
CTOT 728586 complementary to original top strand
CTOB 767899 complementary to original bottom strand
OB 539128 original bottom strand

Deduplication

Alignments analysed 2536630
Unique alignments 384697
Duplicates removed 2151933
Duplicated alignments were found at 182700 different positions

Cytosine Methylation after Extraction

Total C's analysed 2391747
Methylated C's in CpG context 228558
Methylated C's in CHG context 13938
Methylated C's in CHH context 225068
Unmethylated C's in CpG context 102063
Unmethylated C's in CHG context 328800
Unmethylated C's in CHH context 1493320
Percentage methylation (CpG context) 69.1%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 13.1%

M-Bias Plot