Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 42070546
Single-end alignments with a unique best hit 1869213
Sequences without alignments under any condition 38251437
Sequences that did not map uniquely 1949896
Genomic sequence context not extractable (edges of chromosomes) 48

Cytosine Methylation

Total C's analysed 11616286
Methylated C's in CpG context 1919141
Methylated C's in CHG context 67905
Methylated C's in CHH context 604313
Methylated C's in Unknown context 124
Unmethylated C's in CpG context 498929
Unmethylated C's in CHG context 2161381
Unmethylated C's in CHH context 6364617
Unmethylated C's in Unknown context 258
Percentage methylation (CpG context) 79.4%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 8.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 390250 original top strand
CTOT 512015 complementary to original top strand
CTOB 550090 complementary to original bottom strand
OB 416810 original bottom strand

Deduplication

Alignments analysed 1869165
Unique alignments 290788
Duplicates removed 1578377
Duplicated alignments were found at 133346 different positions

Cytosine Methylation after Extraction

Total C's analysed 1825743
Methylated C's in CpG context 202011
Methylated C's in CHG context 12480
Methylated C's in CHH context 193496
Unmethylated C's in CpG context 70791
Unmethylated C's in CHG context 249245
Unmethylated C's in CHH context 1097720
Percentage methylation (CpG context) 74.1%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 15.0%

M-Bias Plot