Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 74531056
Single-end alignments with a unique best hit 3532069
Sequences without alignments under any condition 67728813
Sequences that did not map uniquely 3270174
Genomic sequence context not extractable (edges of chromosomes) 82

Cytosine Methylation

Total C's analysed 21835046
Methylated C's in CpG context 4019431
Methylated C's in CHG context 128117
Methylated C's in CHH context 950616
Methylated C's in Unknown context 97
Unmethylated C's in CpG context 986573
Unmethylated C's in CHG context 3784804
Unmethylated C's in CHH context 11965505
Unmethylated C's in Unknown context 373
Percentage methylation (CpG context) 80.3%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 7.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 758833 original top strand
CTOT 940596 complementary to original top strand
CTOB 1015711 complementary to original bottom strand
OB 816847 original bottom strand

Deduplication

Alignments analysed 3531987
Unique alignments 395118
Duplicates removed 3136869
Duplicated alignments were found at 194707 different positions

Cytosine Methylation after Extraction

Total C's analysed 2439608
Methylated C's in CpG context 263400
Methylated C's in CHG context 16049
Methylated C's in CHH context 234544
Unmethylated C's in CpG context 101061
Unmethylated C's in CHG context 317768
Unmethylated C's in CHH context 1506786
Percentage methylation (CpG context) 72.3%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 13.5%

M-Bias Plot