Alignment Stats
| Sequences analysed in total |
74531056 |
| Single-end alignments with a unique best hit |
3532069 |
| Sequences without alignments under any condition |
67728813 |
| Sequences that did not map uniquely |
3270174 |
| Genomic sequence context not extractable (edges of chromosomes) |
82 |
|
|
Cytosine Methylation
| Total C's analysed |
21835046 |
| Methylated C's in CpG context |
4019431 |
| Methylated C's in CHG context |
128117 |
| Methylated C's in CHH context |
950616 |
| Methylated C's in Unknown context |
97 |
| Unmethylated C's in CpG context |
986573 |
| Unmethylated C's in CHG context |
3784804 |
| Unmethylated C's in CHH context |
11965505 |
| Unmethylated C's in Unknown context |
373 |
| Percentage methylation (CpG context) |
80.3% |
| Percentage methylation (CHG context) |
3.3% |
| Percentage methylation (CHH context) |
7.4% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
758833 |
original top strand |
| CTOT |
940596 |
complementary to original top strand |
| CTOB |
1015711 |
complementary to original bottom strand |
| OB |
816847 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
3531987 |
| Unique alignments |
395118 |
| Duplicates removed |
3136869 |
| Duplicated alignments were found at 194707 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2439608 |
| Methylated C's in CpG context |
263400 |
| Methylated C's in CHG context |
16049 |
| Methylated C's in CHH context |
234544 |
| Unmethylated C's in CpG context |
101061 |
| Unmethylated C's in CHG context |
317768 |
| Unmethylated C's in CHH context |
1506786 |
| Percentage methylation (CpG context) |
72.3% |
| Percentage methylation (CHG context) |
4.8% |
| Percentage methylation (CHH context) |
13.5% |
|
|
M-Bias Plot