Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 67984205
Single-end alignments with a unique best hit 3168995
Sequences without alignments under any condition 61719035
Sequences that did not map uniquely 3096175
Genomic sequence context not extractable (edges of chromosomes) 60

Cytosine Methylation

Total C's analysed 19698902
Methylated C's in CpG context 3595616
Methylated C's in CHG context 120168
Methylated C's in CHH context 904169
Methylated C's in Unknown context 115
Unmethylated C's in CpG context 814267
Unmethylated C's in CHG context 3500880
Unmethylated C's in CHH context 10763802
Unmethylated C's in Unknown context 368
Percentage methylation (CpG context) 81.5%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 7.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 659615 original top strand
CTOT 858524 complementary to original top strand
CTOB 932794 complementary to original bottom strand
OB 718002 original bottom strand

Deduplication

Alignments analysed 3168935
Unique alignments 365699
Duplicates removed 2803236
Duplicated alignments were found at 179085 different positions

Cytosine Methylation after Extraction

Total C's analysed 2275320
Methylated C's in CpG context 252344
Methylated C's in CHG context 15544
Methylated C's in CHH context 227198
Unmethylated C's in CpG context 87619
Unmethylated C's in CHG context 300108
Unmethylated C's in CHH context 1392507
Percentage methylation (CpG context) 74.2%
Percentage methylation (CHG context) 4.9%
Percentage methylation (CHH context) 14.0%

M-Bias Plot