Alignment Stats
| Sequences analysed in total |
67984205 |
| Single-end alignments with a unique best hit |
3168995 |
| Sequences without alignments under any condition |
61719035 |
| Sequences that did not map uniquely |
3096175 |
| Genomic sequence context not extractable (edges of chromosomes) |
60 |
|
|
Cytosine Methylation
| Total C's analysed |
19698902 |
| Methylated C's in CpG context |
3595616 |
| Methylated C's in CHG context |
120168 |
| Methylated C's in CHH context |
904169 |
| Methylated C's in Unknown context |
115 |
| Unmethylated C's in CpG context |
814267 |
| Unmethylated C's in CHG context |
3500880 |
| Unmethylated C's in CHH context |
10763802 |
| Unmethylated C's in Unknown context |
368 |
| Percentage methylation (CpG context) |
81.5% |
| Percentage methylation (CHG context) |
3.3% |
| Percentage methylation (CHH context) |
7.7% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
659615 |
original top strand |
| CTOT |
858524 |
complementary to original top strand |
| CTOB |
932794 |
complementary to original bottom strand |
| OB |
718002 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
3168935 |
| Unique alignments |
365699 |
| Duplicates removed |
2803236 |
| Duplicated alignments were found at 179085 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2275320 |
| Methylated C's in CpG context |
252344 |
| Methylated C's in CHG context |
15544 |
| Methylated C's in CHH context |
227198 |
| Unmethylated C's in CpG context |
87619 |
| Unmethylated C's in CHG context |
300108 |
| Unmethylated C's in CHH context |
1392507 |
| Percentage methylation (CpG context) |
74.2% |
| Percentage methylation (CHG context) |
4.9% |
| Percentage methylation (CHH context) |
14.0% |
|
|
M-Bias Plot