Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 87834637
Single-end alignments with a unique best hit 4288182
Sequences without alignments under any condition 79466280
Sequences that did not map uniquely 4080175
Genomic sequence context not extractable (edges of chromosomes) 80

Cytosine Methylation

Total C's analysed 26352415
Methylated C's in CpG context 4467852
Methylated C's in CHG context 149314
Methylated C's in CHH context 1095506
Methylated C's in Unknown context 120
Unmethylated C's in CpG context 1161113
Unmethylated C's in CHG context 4546966
Unmethylated C's in CHH context 14931664
Unmethylated C's in Unknown context 508
Percentage methylation (CpG context) 79.4%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 6.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 892242 original top strand
CTOT 1163049 complementary to original top strand
CTOB 1266779 complementary to original bottom strand
OB 966032 original bottom strand

Deduplication

Alignments analysed 4288102
Unique alignments 447280
Duplicates removed 3840822
Duplicated alignments were found at 226432 different positions

Cytosine Methylation after Extraction

Total C's analysed 2753570
Methylated C's in CpG context 286266
Methylated C's in CHG context 17092
Methylated C's in CHH context 251098
Unmethylated C's in CpG context 112046
Unmethylated C's in CHG context 356809
Unmethylated C's in CHH context 1730259
Percentage methylation (CpG context) 71.9%
Percentage methylation (CHG context) 4.6%
Percentage methylation (CHH context) 12.7%

M-Bias Plot