Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/v082/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 64352550 Number of alignments with a unique best hit from the different alignments: 3006813 Mapping efficiency: 4.7% Sequences with no alignments under any condition: 58253464 Sequences did not map uniquely: 3092273 Sequences which were discarded because genomic sequence could not be extracted: 83 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 613488 ((converted) top strand) CT/GA: 669500 ((converted) bottom strand) GA/CT: 827743 (complementary to (converted) top strand) GA/GA: 895999 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 18725396 Total methylated C's in CpG context: 3032628 Total methylated C's in CHG context: 105154 Total methylated C's in CHH context: 863947 Total methylated C's in Unknown context: 102 Total unmethylated C's in CpG context: 833340 Total unmethylated C's in CHG context: 3335556 Total unmethylated C's in CHH context: 10554771 Total unmethylated C's in Unknown context: 418 C methylated in CpG context: 78.4% C methylated in CHG context: 3.1% C methylated in CHH context: 7.6% C methylated in Unknown context (CN or CHN): 19.6% Bismark completed in 0d 0h 33m 9s