Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 64352550
Single-end alignments with a unique best hit 3006813
Sequences without alignments under any condition 58253464
Sequences that did not map uniquely 3092273
Genomic sequence context not extractable (edges of chromosomes) 83

Cytosine Methylation

Total C's analysed 18725396
Methylated C's in CpG context 3032628
Methylated C's in CHG context 105154
Methylated C's in CHH context 863947
Methylated C's in Unknown context 102
Unmethylated C's in CpG context 833340
Unmethylated C's in CHG context 3335556
Unmethylated C's in CHH context 10554771
Unmethylated C's in Unknown context 418
Percentage methylation (CpG context) 78.4%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 7.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 613488 original top strand
CTOT 827743 complementary to original top strand
CTOB 895999 complementary to original bottom strand
OB 669500 original bottom strand

Deduplication

Alignments analysed 3006730
Unique alignments 371361
Duplicates removed 2635369
Duplicated alignments were found at 183029 different positions

Cytosine Methylation after Extraction

Total C's analysed 2317028
Methylated C's in CpG context 246179
Methylated C's in CHG context 15000
Methylated C's in CHH context 227786
Unmethylated C's in CpG context 93775
Unmethylated C's in CHG context 307464
Unmethylated C's in CHH context 1426824
Percentage methylation (CpG context) 72.4%
Percentage methylation (CHG context) 4.7%
Percentage methylation (CHH context) 13.8%

M-Bias Plot