Alignment Stats
| Sequences analysed in total |
64352550 |
| Single-end alignments with a unique best hit |
3006813 |
| Sequences without alignments under any condition |
58253464 |
| Sequences that did not map uniquely |
3092273 |
| Genomic sequence context not extractable (edges of chromosomes) |
83 |
|
|
Cytosine Methylation
| Total C's analysed |
18725396 |
| Methylated C's in CpG context |
3032628 |
| Methylated C's in CHG context |
105154 |
| Methylated C's in CHH context |
863947 |
| Methylated C's in Unknown context |
102 |
| Unmethylated C's in CpG context |
833340 |
| Unmethylated C's in CHG context |
3335556 |
| Unmethylated C's in CHH context |
10554771 |
| Unmethylated C's in Unknown context |
418 |
| Percentage methylation (CpG context) |
78.4% |
| Percentage methylation (CHG context) |
3.1% |
| Percentage methylation (CHH context) |
7.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
613488 |
original top strand |
| CTOT |
827743 |
complementary to original top strand |
| CTOB |
895999 |
complementary to original bottom strand |
| OB |
669500 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
3006730 |
| Unique alignments |
371361 |
| Duplicates removed |
2635369 |
| Duplicated alignments were found at 183029 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2317028 |
| Methylated C's in CpG context |
246179 |
| Methylated C's in CHG context |
15000 |
| Methylated C's in CHH context |
227786 |
| Unmethylated C's in CpG context |
93775 |
| Unmethylated C's in CHG context |
307464 |
| Unmethylated C's in CHH context |
1426824 |
| Percentage methylation (CpG context) |
72.4% |
| Percentage methylation (CHG context) |
4.7% |
| Percentage methylation (CHH context) |
13.8% |
|
|
M-Bias Plot