Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 2728328
Sequences without alignments under any condition 53907751
Sequences that did not map uniquely 2823352
Genomic sequence context not extractable (edges of chromosomes) 84

Cytosine Methylation

Total C's analysed 16759013
Methylated C's in CpG context 1970106
Methylated C's in CHG context 81593
Methylated C's in CHH context 852411
Methylated C's in Unknown context 54
Unmethylated C's in CpG context 816317
Unmethylated C's in CHG context 3000835
Unmethylated C's in CHH context 10037751
Unmethylated C's in Unknown context 490
Percentage methylation (CpG context) 70.7%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 7.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 555991 original top strand
CTOT 756652 complementary to original top strand
CTOB 813523 complementary to original bottom strand
OB 602078 original bottom strand

Deduplication

Alignments analysed 2728244
Unique alignments 417365
Duplicates removed 2310879
Duplicated alignments were found at 193445 different positions

Cytosine Methylation after Extraction

Total C's analysed 2575743
Methylated C's in CpG context 226998
Methylated C's in CHG context 14505
Methylated C's in CHH context 249861
Unmethylated C's in CpG context 117529
Unmethylated C's in CHG context 349443
Unmethylated C's in CHH context 1617407
Percentage methylation (CpG context) 65.9%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 13.4%

M-Bias Plot