Alignment Stats
| Sequences analysed in total |
59459431 |
| Single-end alignments with a unique best hit |
2728328 |
| Sequences without alignments under any condition |
53907751 |
| Sequences that did not map uniquely |
2823352 |
| Genomic sequence context not extractable (edges of chromosomes) |
84 |
|
|
Cytosine Methylation
| Total C's analysed |
16759013 |
| Methylated C's in CpG context |
1970106 |
| Methylated C's in CHG context |
81593 |
| Methylated C's in CHH context |
852411 |
| Methylated C's in Unknown context |
54 |
| Unmethylated C's in CpG context |
816317 |
| Unmethylated C's in CHG context |
3000835 |
| Unmethylated C's in CHH context |
10037751 |
| Unmethylated C's in Unknown context |
490 |
| Percentage methylation (CpG context) |
70.7% |
| Percentage methylation (CHG context) |
2.6% |
| Percentage methylation (CHH context) |
7.8% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
555991 |
original top strand |
| CTOT |
756652 |
complementary to original top strand |
| CTOB |
813523 |
complementary to original bottom strand |
| OB |
602078 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
2728244 |
| Unique alignments |
417365 |
| Duplicates removed |
2310879 |
| Duplicated alignments were found at 193445 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
2575743 |
| Methylated C's in CpG context |
226998 |
| Methylated C's in CHG context |
14505 |
| Methylated C's in CHH context |
249861 |
| Unmethylated C's in CpG context |
117529 |
| Unmethylated C's in CHG context |
349443 |
| Unmethylated C's in CHH context |
1617407 |
| Percentage methylation (CpG context) |
65.9% |
| Percentage methylation (CHG context) |
4.0% |
| Percentage methylation (CHH context) |
13.4% |
|
|
M-Bias Plot