Alignment Stats
| Sequences analysed in total |
54458572 |
| Single-end alignments with a unique best hit |
2533316 |
| Sequences without alignments under any condition |
49708344 |
| Sequences that did not map uniquely |
2216912 |
| Genomic sequence context not extractable (edges of chromosomes) |
60 |
|
|
Cytosine Methylation
| Total C's analysed |
15943045 |
| Methylated C's in CpG context |
3143225 |
| Methylated C's in CHG context |
96153 |
| Methylated C's in CHH context |
702171 |
| Methylated C's in Unknown context |
141 |
| Unmethylated C's in CpG context |
717521 |
| Unmethylated C's in CHG context |
2746405 |
| Unmethylated C's in CHH context |
8537570 |
| Unmethylated C's in Unknown context |
281 |
| Percentage methylation (CpG context) |
81.4% |
| Percentage methylation (CHG context) |
3.4% |
| Percentage methylation (CHH context) |
7.6% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
526236 |
original top strand |
| CTOT |
693851 |
complementary to original top strand |
| CTOB |
742811 |
complementary to original bottom strand |
| OB |
570358 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
2533256 |
| Unique alignments |
304671 |
| Duplicates removed |
2228585 |
| Duplicated alignments were found at 144132 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1903561 |
| Methylated C's in CpG context |
205058 |
| Methylated C's in CHG context |
12613 |
| Methylated C's in CHH context |
183446 |
| Unmethylated C's in CpG context |
85540 |
| Unmethylated C's in CHG context |
251339 |
| Unmethylated C's in CHH context |
1165565 |
| Percentage methylation (CpG context) |
70.6% |
| Percentage methylation (CHG context) |
4.8% |
| Percentage methylation (CHH context) |
13.6% |
|
|
M-Bias Plot