Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_11_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 54458572
Single-end alignments with a unique best hit 2533316
Sequences without alignments under any condition 49708344
Sequences that did not map uniquely 2216912
Genomic sequence context not extractable (edges of chromosomes) 60

Cytosine Methylation

Total C's analysed 15943045
Methylated C's in CpG context 3143225
Methylated C's in CHG context 96153
Methylated C's in CHH context 702171
Methylated C's in Unknown context 141
Unmethylated C's in CpG context 717521
Unmethylated C's in CHG context 2746405
Unmethylated C's in CHH context 8537570
Unmethylated C's in Unknown context 281
Percentage methylation (CpG context) 81.4%
Percentage methylation (CHG context) 3.4%
Percentage methylation (CHH context) 7.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 526236 original top strand
CTOT 693851 complementary to original top strand
CTOB 742811 complementary to original bottom strand
OB 570358 original bottom strand

Deduplication

Alignments analysed 2533256
Unique alignments 304671
Duplicates removed 2228585
Duplicated alignments were found at 144132 different positions

Cytosine Methylation after Extraction

Total C's analysed 1903561
Methylated C's in CpG context 205058
Methylated C's in CHG context 12613
Methylated C's in CHH context 183446
Unmethylated C's in CpG context 85540
Unmethylated C's in CHG context 251339
Unmethylated C's in CHH context 1165565
Percentage methylation (CpG context) 70.6%
Percentage methylation (CHG context) 4.8%
Percentage methylation (CHH context) 13.6%

M-Bias Plot