Alignment Stats
| Sequences analysed in total |
78221457 |
| Single-end alignments with a unique best hit |
3147226 |
| Sequences without alignments under any condition |
71822548 |
| Sequences that did not map uniquely |
3251683 |
| Genomic sequence context not extractable (edges of chromosomes) |
75 |
|
|
Cytosine Methylation
| Total C's analysed |
19635772 |
| Methylated C's in CpG context |
2906053 |
| Methylated C's in CHG context |
106168 |
| Methylated C's in CHH context |
1071747 |
| Methylated C's in Unknown context |
120 |
| Unmethylated C's in CpG context |
877521 |
| Unmethylated C's in CHG context |
3583731 |
| Unmethylated C's in CHH context |
11090552 |
| Unmethylated C's in Unknown context |
424 |
| Percentage methylation (CpG context) |
76.8% |
| Percentage methylation (CHG context) |
2.9% |
| Percentage methylation (CHH context) |
8.8% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
763339 |
original top strand |
| CTOT |
766511 |
complementary to original top strand |
| CTOB |
808837 |
complementary to original bottom strand |
| OB |
808464 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
3147151 |
| Unique alignments |
310130 |
| Duplicates removed |
2837021 |
| Duplicated alignments were found at 175832 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1940126 |
| Methylated C's in CpG context |
184224 |
| Methylated C's in CHG context |
13483 |
| Methylated C's in CHH context |
221285 |
| Unmethylated C's in CpG context |
81087 |
| Unmethylated C's in CHG context |
249490 |
| Unmethylated C's in CHH context |
1190557 |
| Percentage methylation (CpG context) |
69.4% |
| Percentage methylation (CHG context) |
5.1% |
| Percentage methylation (CHH context) |
15.7% |
|
|
M-Bias Plot