Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_10_s456_trimmed.fq

Data processed at 01:38 on 2022-06-18


Alignment Stats

Sequences analysed in total 78221457
Single-end alignments with a unique best hit 3147226
Sequences without alignments under any condition 71822548
Sequences that did not map uniquely 3251683
Genomic sequence context not extractable (edges of chromosomes) 75

Cytosine Methylation

Total C's analysed 19635772
Methylated C's in CpG context 2906053
Methylated C's in CHG context 106168
Methylated C's in CHH context 1071747
Methylated C's in Unknown context 120
Unmethylated C's in CpG context 877521
Unmethylated C's in CHG context 3583731
Unmethylated C's in CHH context 11090552
Unmethylated C's in Unknown context 424
Percentage methylation (CpG context) 76.8%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 8.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 763339 original top strand
CTOT 766511 complementary to original top strand
CTOB 808837 complementary to original bottom strand
OB 808464 original bottom strand

Deduplication

Alignments analysed 3147151
Unique alignments 310130
Duplicates removed 2837021
Duplicated alignments were found at 175832 different positions

Cytosine Methylation after Extraction

Total C's analysed 1940126
Methylated C's in CpG context 184224
Methylated C's in CHG context 13483
Methylated C's in CHH context 221285
Unmethylated C's in CpG context 81087
Unmethylated C's in CHG context 249490
Unmethylated C's in CHH context 1190557
Percentage methylation (CpG context) 69.4%
Percentage methylation (CHG context) 5.1%
Percentage methylation (CHH context) 15.7%

M-Bias Plot