Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_all_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 605809374
Single-end alignments with a unique best hit 532554
Sequences without alignments under any condition 604756951
Sequences that did not map uniquely 519869
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 4742693
Methylated C's in CpG context 129843
Methylated C's in CHG context 74534
Methylated C's in CHH context 1017146
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 163526
Unmethylated C's in CHG context 332272
Unmethylated C's in CHH context 3025372
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 44.3%
Percentage methylation (CHG context) 18.3%
Percentage methylation (CHH context) 25.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 132066 original top strand
CTOT 170121 complementary to original top strand
CTOB 132376 complementary to original bottom strand
OB 97991 original bottom strand

Deduplication

Alignments analysed 532554
Unique alignments 140425
Duplicates removed 392129
Duplicated alignments were found at 40399 different positions

Cytosine Methylation after Extraction

Total C's analysed 988380
Methylated C's in CpG context 50777
Methylated C's in CHG context 20676
Methylated C's in CHH context 183159
Unmethylated C's in CpG context 73873
Unmethylated C's in CHG context 105337
Unmethylated C's in CHH context 554558
Percentage methylation (CpG context) 40.7%
Percentage methylation (CHG context) 16.4%
Percentage methylation (CHH context) 24.8%

M-Bias Plot