Alignment Stats
| Sequences analysed in total |
77350922 |
| Single-end alignments with a unique best hit |
71985 |
| Sequences without alignments under any condition |
77197216 |
| Sequences that did not map uniquely |
81721 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
657760 |
| Methylated C's in CpG context |
12433 |
| Methylated C's in CHG context |
8567 |
| Methylated C's in CHH context |
133046 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
21781 |
| Unmethylated C's in CHG context |
43876 |
| Unmethylated C's in CHH context |
438057 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
36.3% |
| Percentage methylation (CHG context) |
16.3% |
| Percentage methylation (CHH context) |
23.3% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
17891 |
original top strand |
| CTOT |
23688 |
complementary to original top strand |
| CTOB |
17466 |
complementary to original bottom strand |
| OB |
12940 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
71985 |
| Unique alignments |
28224 |
| Duplicates removed |
43761 |
| Duplicated alignments were found at 6026 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
209889 |
| Methylated C's in CpG context |
7438 |
| Methylated C's in CHG context |
3438 |
| Methylated C's in CHH context |
42191 |
| Unmethylated C's in CpG context |
12857 |
| Unmethylated C's in CHG context |
18335 |
| Unmethylated C's in CHH context |
125630 |
| Percentage methylation (CpG context) |
36.6% |
| Percentage methylation (CHG context) |
15.8% |
| Percentage methylation (CHH context) |
25.1% |
|
|
M-Bias Plot