Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_9_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 77350922
Single-end alignments with a unique best hit 71985
Sequences without alignments under any condition 77197216
Sequences that did not map uniquely 81721
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 657760
Methylated C's in CpG context 12433
Methylated C's in CHG context 8567
Methylated C's in CHH context 133046
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 21781
Unmethylated C's in CHG context 43876
Unmethylated C's in CHH context 438057
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 36.3%
Percentage methylation (CHG context) 16.3%
Percentage methylation (CHH context) 23.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 17891 original top strand
CTOT 23688 complementary to original top strand
CTOB 17466 complementary to original bottom strand
OB 12940 original bottom strand

Deduplication

Alignments analysed 71985
Unique alignments 28224
Duplicates removed 43761
Duplicated alignments were found at 6026 different positions

Cytosine Methylation after Extraction

Total C's analysed 209889
Methylated C's in CpG context 7438
Methylated C's in CHG context 3438
Methylated C's in CHH context 42191
Unmethylated C's in CpG context 12857
Unmethylated C's in CHG context 18335
Unmethylated C's in CHH context 125630
Percentage methylation (CpG context) 36.6%
Percentage methylation (CHG context) 15.8%
Percentage methylation (CHH context) 25.1%

M-Bias Plot