Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_8_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 38697189
Single-end alignments with a unique best hit 37282
Sequences without alignments under any condition 38620922
Sequences that did not map uniquely 38985
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 343855
Methylated C's in CpG context 8361
Methylated C's in CHG context 5877
Methylated C's in CHH context 81599
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 10741
Unmethylated C's in CHG context 22312
Unmethylated C's in CHH context 214965
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 43.8%
Percentage methylation (CHG context) 20.8%
Percentage methylation (CHH context) 27.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 8957 original top strand
CTOT 12481 complementary to original top strand
CTOB 9323 complementary to original bottom strand
OB 6520 original bottom strand

Deduplication

Alignments analysed 37281
Unique alignments 16682
Duplicates removed 20599
Duplicated alignments were found at 3159 different positions

Cytosine Methylation after Extraction

Total C's analysed 127461
Methylated C's in CpG context 5382
Methylated C's in CHG context 2475
Methylated C's in CHH context 27803
Unmethylated C's in CpG context 7089
Unmethylated C's in CHG context 10749
Unmethylated C's in CHH context 73963
Percentage methylation (CpG context) 43.2%
Percentage methylation (CHG context) 18.7%
Percentage methylation (CHH context) 27.3%

M-Bias Plot