Alignment Stats
| Sequences analysed in total |
46455148 |
| Single-end alignments with a unique best hit |
43915 |
| Sequences without alignments under any condition |
46365550 |
| Sequences that did not map uniquely |
45683 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
406252 |
| Methylated C's in CpG context |
7382 |
| Methylated C's in CHG context |
6380 |
| Methylated C's in CHH context |
88839 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
13797 |
| Unmethylated C's in CHG context |
26392 |
| Unmethylated C's in CHH context |
263462 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
34.9% |
| Percentage methylation (CHG context) |
19.5% |
| Percentage methylation (CHH context) |
25.2% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
10896 |
original top strand |
| CTOT |
14577 |
complementary to original top strand |
| CTOB |
10439 |
complementary to original bottom strand |
| OB |
8003 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
43915 |
| Unique alignments |
19043 |
| Duplicates removed |
24872 |
| Duplicated alignments were found at 3834 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
145821 |
| Methylated C's in CpG context |
4773 |
| Methylated C's in CHG context |
2601 |
| Methylated C's in CHH context |
30565 |
| Unmethylated C's in CpG context |
8768 |
| Unmethylated C's in CHG context |
12179 |
| Unmethylated C's in CHH context |
86935 |
| Percentage methylation (CpG context) |
35.2% |
| Percentage methylation (CHG context) |
17.6% |
| Percentage methylation (CHH context) |
26.0% |
|
|
M-Bias Plot