Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_6_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 46455148
Single-end alignments with a unique best hit 43915
Sequences without alignments under any condition 46365550
Sequences that did not map uniquely 45683
Genomic sequence context not extractable (edges of chromosomes) 0
0.0945%99.8%0.0983%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 406252
Methylated C's in CpG context 7382
Methylated C's in CHG context 6380
Methylated C's in CHH context 88839
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 13797
Unmethylated C's in CHG context 26392
Unmethylated C's in CHH context 263462
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 34.9%
Percentage methylation (CHG context) 19.5%
Percentage methylation (CHH context) 25.2%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 10896 original top strand
CTOT 14577 complementary to original top strand
CTOB 10439 complementary to original bottom strand
OB 8003 original bottom strand
OTCTOTCTOBOB02k4k6k8k10k12k14k
Number of Alignments

Deduplication

Alignments analysed 43915
Unique alignments 19043
Duplicates removed 24872
Duplicated alignments were found at 3834 different positions
43.4%56.6%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 145821
Methylated C's in CpG context 4773
Methylated C's in CHG context 2601
Methylated C's in CHH context 30565
Unmethylated C's in CpG context 8768
Unmethylated C's in CHG context 12179
Unmethylated C's in CHH context 86935
Percentage methylation (CpG context) 35.2%
Percentage methylation (CHG context) 17.6%
Percentage methylation (CHH context) 26.0%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

51015202530020406080100010002000300040005000
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls