Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_5_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 47401736
Single-end alignments with a unique best hit 49826
Sequences without alignments under any condition 47302825
Sequences that did not map uniquely 49085
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 450928
Methylated C's in CpG context 8978
Methylated C's in CHG context 7241
Methylated C's in CHH context 111161
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 15009
Unmethylated C's in CHG context 28241
Unmethylated C's in CHH context 280298
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 37.4%
Percentage methylation (CHG context) 20.4%
Percentage methylation (CHH context) 28.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11986 original top strand
CTOT 16666 complementary to original top strand
CTOB 12304 complementary to original bottom strand
OB 8870 original bottom strand

Deduplication

Alignments analysed 49826
Unique alignments 22195
Duplicates removed 27631
Duplicated alignments were found at 4367 different positions

Cytosine Methylation after Extraction

Total C's analysed 165255
Methylated C's in CpG context 5787
Methylated C's in CHG context 3061
Methylated C's in CHH context 38005
Unmethylated C's in CpG context 9997
Unmethylated C's in CHG context 13748
Unmethylated C's in CHH context 94657
Percentage methylation (CpG context) 36.7%
Percentage methylation (CHG context) 18.2%
Percentage methylation (CHH context) 28.6%

M-Bias Plot