Alignment Stats
| Sequences analysed in total |
47401736 |
| Single-end alignments with a unique best hit |
49826 |
| Sequences without alignments under any condition |
47302825 |
| Sequences that did not map uniquely |
49085 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
450928 |
| Methylated C's in CpG context |
8978 |
| Methylated C's in CHG context |
7241 |
| Methylated C's in CHH context |
111161 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
15009 |
| Unmethylated C's in CHG context |
28241 |
| Unmethylated C's in CHH context |
280298 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
37.4% |
| Percentage methylation (CHG context) |
20.4% |
| Percentage methylation (CHH context) |
28.4% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
11986 |
original top strand |
| CTOT |
16666 |
complementary to original top strand |
| CTOB |
12304 |
complementary to original bottom strand |
| OB |
8870 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
49826 |
| Unique alignments |
22195 |
| Duplicates removed |
27631 |
| Duplicated alignments were found at 4367 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
165255 |
| Methylated C's in CpG context |
5787 |
| Methylated C's in CHG context |
3061 |
| Methylated C's in CHH context |
38005 |
| Unmethylated C's in CpG context |
9997 |
| Unmethylated C's in CHG context |
13748 |
| Unmethylated C's in CHH context |
94657 |
| Percentage methylation (CpG context) |
36.7% |
| Percentage methylation (CHG context) |
18.2% |
| Percentage methylation (CHH context) |
28.6% |
|
|
M-Bias Plot