Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_4_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 44905078
Single-end alignments with a unique best hit 42602
Sequences without alignments under any condition 44819761
Sequences that did not map uniquely 42715
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 389304
Methylated C's in CpG context 9645
Methylated C's in CHG context 6539
Methylated C's in CHH context 89476
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 12460
Unmethylated C's in CHG context 25751
Unmethylated C's in CHH context 245433
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 43.6%
Percentage methylation (CHG context) 20.3%
Percentage methylation (CHH context) 26.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 10714 original top strand
CTOT 13768 complementary to original top strand
CTOB 10383 complementary to original bottom strand
OB 7737 original bottom strand

Deduplication

Alignments analysed 42602
Unique alignments 18809
Duplicates removed 23793
Duplicated alignments were found at 3597 different positions

Cytosine Methylation after Extraction

Total C's analysed 142595
Methylated C's in CpG context 6220
Methylated C's in CHG context 2848
Methylated C's in CHH context 31126
Unmethylated C's in CpG context 8211
Unmethylated C's in CHG context 12177
Unmethylated C's in CHH context 82013
Percentage methylation (CpG context) 43.1%
Percentage methylation (CHG context) 19.0%
Percentage methylation (CHH context) 27.5%

M-Bias Plot