Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_3_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 48376729
Single-end alignments with a unique best hit 45097
Sequences without alignments under any condition 48284321
Sequences that did not map uniquely 47311
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 411499
Methylated C's in CpG context 9538
Methylated C's in CHG context 6638
Methylated C's in CHH context 92034
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 13583
Unmethylated C's in CHG context 27231
Unmethylated C's in CHH context 262475
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 41.3%
Percentage methylation (CHG context) 19.6%
Percentage methylation (CHH context) 26.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11274 original top strand
CTOT 14541 complementary to original top strand
CTOB 10931 complementary to original bottom strand
OB 8351 original bottom strand

Deduplication

Alignments analysed 45097
Unique alignments 20093
Duplicates removed 25004
Duplicated alignments were found at 3790 different positions

Cytosine Methylation after Extraction

Total C's analysed 152187
Methylated C's in CpG context 6120
Methylated C's in CHG context 2843
Methylated C's in CHH context 31545
Unmethylated C's in CpG context 9099
Unmethylated C's in CHG context 13065
Unmethylated C's in CHH context 89515
Percentage methylation (CpG context) 40.2%
Percentage methylation (CHG context) 17.9%
Percentage methylation (CHH context) 26.1%

M-Bias Plot