Alignment Stats
| Sequences analysed in total |
48376729 |
| Single-end alignments with a unique best hit |
45097 |
| Sequences without alignments under any condition |
48284321 |
| Sequences that did not map uniquely |
47311 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
411499 |
| Methylated C's in CpG context |
9538 |
| Methylated C's in CHG context |
6638 |
| Methylated C's in CHH context |
92034 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
13583 |
| Unmethylated C's in CHG context |
27231 |
| Unmethylated C's in CHH context |
262475 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
41.3% |
| Percentage methylation (CHG context) |
19.6% |
| Percentage methylation (CHH context) |
26.0% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
11274 |
original top strand |
| CTOT |
14541 |
complementary to original top strand |
| CTOB |
10931 |
complementary to original bottom strand |
| OB |
8351 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
45097 |
| Unique alignments |
20093 |
| Duplicates removed |
25004 |
| Duplicated alignments were found at 3790 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
152187 |
| Methylated C's in CpG context |
6120 |
| Methylated C's in CHG context |
2843 |
| Methylated C's in CHH context |
31545 |
| Unmethylated C's in CpG context |
9099 |
| Unmethylated C's in CHG context |
13065 |
| Unmethylated C's in CHH context |
89515 |
| Percentage methylation (CpG context) |
40.2% |
| Percentage methylation (CHG context) |
17.9% |
| Percentage methylation (CHH context) |
26.1% |
|
|
M-Bias Plot