Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_2_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 46105140
Single-end alignments with a unique best hit 45684
Sequences without alignments under any condition 46014221
Sequences that did not map uniquely 45235
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 413360
Methylated C's in CpG context 9582
Methylated C's in CHG context 7044
Methylated C's in CHH context 101882
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 13142
Unmethylated C's in CHG context 26557
Unmethylated C's in CHH context 255153
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 42.2%
Percentage methylation (CHG context) 21.0%
Percentage methylation (CHH context) 28.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11348 original top strand
CTOT 14951 complementary to original top strand
CTOB 11190 complementary to original bottom strand
OB 8195 original bottom strand

Deduplication

Alignments analysed 45684
Unique alignments 19947
Duplicates removed 25737
Duplicated alignments were found at 4090 different positions

Cytosine Methylation after Extraction

Total C's analysed 149958
Methylated C's in CpG context 5979
Methylated C's in CHG context 2972
Methylated C's in CHH context 33824
Unmethylated C's in CpG context 8723
Unmethylated C's in CHG context 12369
Unmethylated C's in CHH context 86091
Percentage methylation (CpG context) 40.7%
Percentage methylation (CHG context) 19.4%
Percentage methylation (CHH context) 28.2%

M-Bias Plot