Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_1_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 46792286
Single-end alignments with a unique best hit 44887
Sequences without alignments under any condition 46701591
Sequences that did not map uniquely 45808
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 405975
Methylated C's in CpG context 9744
Methylated C's in CHG context 6987
Methylated C's in CHH context 93377
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 13251
Unmethylated C's in CHG context 26590
Unmethylated C's in CHH context 256026
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 42.4%
Percentage methylation (CHG context) 20.8%
Percentage methylation (CHH context) 26.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 10930 original top strand
CTOT 14550 complementary to original top strand
CTOB 11130 complementary to original bottom strand
OB 8277 original bottom strand

Deduplication

Alignments analysed 44887
Unique alignments 20374
Duplicates removed 24513
Duplicated alignments were found at 3925 different positions

Cytosine Methylation after Extraction

Total C's analysed 152754
Methylated C's in CpG context 6352
Methylated C's in CHG context 3158
Methylated C's in CHH context 33728
Unmethylated C's in CpG context 8815
Unmethylated C's in CHG context 12825
Unmethylated C's in CHH context 87876
Percentage methylation (CpG context) 41.9%
Percentage methylation (CHG context) 19.8%
Percentage methylation (CHH context) 27.7%

M-Bias Plot