Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_17_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 42070546
Single-end alignments with a unique best hit 41080
Sequences without alignments under any condition 41994231
Sequences that did not map uniquely 35235
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 356764
Methylated C's in CpG context 10515
Methylated C's in CHG context 6264
Methylated C's in CHH context 87134
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 12210
Unmethylated C's in CHG context 24224
Unmethylated C's in CHH context 216417
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 46.3%
Percentage methylation (CHG context) 20.5%
Percentage methylation (CHH context) 28.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 10192 original top strand
CTOT 12778 complementary to original top strand
CTOB 10256 complementary to original bottom strand
OB 7854 original bottom strand

Deduplication

Alignments analysed 41080
Unique alignments 20003
Duplicates removed 21077
Duplicated alignments were found at 3820 different positions

Cytosine Methylation after Extraction

Total C's analysed 147288
Methylated C's in CpG context 6935
Methylated C's in CHG context 3064
Methylated C's in CHH context 34116
Unmethylated C's in CpG context 8570
Unmethylated C's in CHG context 12727
Unmethylated C's in CHH context 81876
Percentage methylation (CpG context) 44.7%
Percentage methylation (CHG context) 19.4%
Percentage methylation (CHH context) 29.4%

M-Bias Plot