Alignment Stats
| Sequences analysed in total |
74531056 |
| Single-end alignments with a unique best hit |
63096 |
| Sequences without alignments under any condition |
74404709 |
| Sequences that did not map uniquely |
63251 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
558695 |
| Methylated C's in CpG context |
16677 |
| Methylated C's in CHG context |
8842 |
| Methylated C's in CHH context |
115582 |
| Methylated C's in Unknown context |
0 |
| Unmethylated C's in CpG context |
19192 |
| Unmethylated C's in CHG context |
40227 |
| Unmethylated C's in CHH context |
358175 |
| Unmethylated C's in Unknown context |
0 |
| Percentage methylation (CpG context) |
46.5% |
| Percentage methylation (CHG context) |
18.0% |
| Percentage methylation (CHH context) |
24.4% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
16195 |
original top strand |
| CTOT |
19769 |
complementary to original top strand |
| CTOB |
15241 |
complementary to original bottom strand |
| OB |
11891 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
63096 |
| Unique alignments |
27663 |
| Duplicates removed |
35433 |
| Duplicated alignments were found at 5410 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
203654 |
| Methylated C's in CpG context |
10065 |
| Methylated C's in CHG context |
4084 |
| Methylated C's in CHH context |
40789 |
| Unmethylated C's in CpG context |
12148 |
| Unmethylated C's in CHG context |
18587 |
| Unmethylated C's in CHH context |
117981 |
| Percentage methylation (CpG context) |
45.3% |
| Percentage methylation (CHG context) |
18.0% |
| Percentage methylation (CHH context) |
25.7% |
|
|
M-Bias Plot