Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_16_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 74531056
Single-end alignments with a unique best hit 63096
Sequences without alignments under any condition 74404709
Sequences that did not map uniquely 63251
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 558695
Methylated C's in CpG context 16677
Methylated C's in CHG context 8842
Methylated C's in CHH context 115582
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 19192
Unmethylated C's in CHG context 40227
Unmethylated C's in CHH context 358175
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 46.5%
Percentage methylation (CHG context) 18.0%
Percentage methylation (CHH context) 24.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 16195 original top strand
CTOT 19769 complementary to original top strand
CTOB 15241 complementary to original bottom strand
OB 11891 original bottom strand

Deduplication

Alignments analysed 63096
Unique alignments 27663
Duplicates removed 35433
Duplicated alignments were found at 5410 different positions

Cytosine Methylation after Extraction

Total C's analysed 203654
Methylated C's in CpG context 10065
Methylated C's in CHG context 4084
Methylated C's in CHH context 40789
Unmethylated C's in CpG context 12148
Unmethylated C's in CHG context 18587
Unmethylated C's in CHH context 117981
Percentage methylation (CpG context) 45.3%
Percentage methylation (CHG context) 18.0%
Percentage methylation (CHH context) 25.7%

M-Bias Plot