Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq (version: v0.21.0) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/ecoli-MG1655/ with the specified options: -q --score-min L,0,-0.2 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 67984205 Number of alignments with a unique best hit from the different alignments: 60150 Mapping efficiency: 0.1% Sequences with no alignments under any condition: 67866144 Sequences did not map uniquely: 57911 Sequences which were discarded because genomic sequence could not be extracted: 0 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 15232 ((converted) top strand) CT/GA: 10815 ((converted) bottom strand) GA/CT: 19424 (complementary to (converted) top strand) GA/GA: 14679 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 537349 Total methylated C's in CpG context: 16572 Total methylated C's in CHG context: 9050 Total methylated C's in CHH context: 119627 Total methylated C's in Unknown context: 0 Total unmethylated C's in CpG context: 18189 Total unmethylated C's in CHG context: 37658 Total unmethylated C's in CHH context: 336253 Total unmethylated C's in Unknown context: 0 C methylated in CpG context: 47.7% C methylated in CHG context: 19.4% C methylated in CHH context: 26.2% Can't determine percentage of methylated Cs in Unknown context (CN or CHN) if value was 0 Bismark completed in 0d 0h 26m 53s