Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_15_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 67984205
Single-end alignments with a unique best hit 60150
Sequences without alignments under any condition 67866144
Sequences that did not map uniquely 57911
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 537349
Methylated C's in CpG context 16572
Methylated C's in CHG context 9050
Methylated C's in CHH context 119627
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 18189
Unmethylated C's in CHG context 37658
Unmethylated C's in CHH context 336253
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 47.7%
Percentage methylation (CHG context) 19.4%
Percentage methylation (CHH context) 26.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15232 original top strand
CTOT 19424 complementary to original top strand
CTOB 14679 complementary to original bottom strand
OB 10815 original bottom strand

Deduplication

Alignments analysed 60150
Unique alignments 26337
Duplicates removed 33813
Duplicated alignments were found at 5132 different positions

Cytosine Methylation after Extraction

Total C's analysed 194637
Methylated C's in CpG context 9811
Methylated C's in CHG context 4057
Methylated C's in CHH context 40377
Unmethylated C's in CpG context 11554
Unmethylated C's in CHG context 17815
Unmethylated C's in CHH context 111023
Percentage methylation (CpG context) 45.9%
Percentage methylation (CHG context) 18.5%
Percentage methylation (CHH context) 26.7%

M-Bias Plot