Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_14_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 87834637
Single-end alignments with a unique best hit 73054
Sequences without alignments under any condition 87680345
Sequences that did not map uniquely 81238
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 662534
Methylated C's in CpG context 17289
Methylated C's in CHG context 9397
Methylated C's in CHH context 123380
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 22945
Unmethylated C's in CHG context 47531
Unmethylated C's in CHH context 441992
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 43.0%
Percentage methylation (CHG context) 16.5%
Percentage methylation (CHH context) 21.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 18485 original top strand
CTOT 23389 complementary to original top strand
CTOB 17632 complementary to original bottom strand
OB 13548 original bottom strand

Deduplication

Alignments analysed 73054
Unique alignments 29797
Duplicates removed 43257
Duplicated alignments were found at 5889 different positions

Cytosine Methylation after Extraction

Total C's analysed 222251
Methylated C's in CpG context 10111
Methylated C's in CHG context 4142
Methylated C's in CHH context 41177
Unmethylated C's in CpG context 13872
Unmethylated C's in CHG context 20535
Unmethylated C's in CHH context 132414
Percentage methylation (CpG context) 42.2%
Percentage methylation (CHG context) 16.8%
Percentage methylation (CHH context) 23.7%

M-Bias Plot