Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_13_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 64352550
Single-end alignments with a unique best hit 57456
Sequences without alignments under any condition 64237333
Sequences that did not map uniquely 57761
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 515655
Methylated C's in CpG context 13921
Methylated C's in CHG context 8111
Methylated C's in CHH context 110523
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 17473
Unmethylated C's in CHG context 36139
Unmethylated C's in CHH context 329488
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 44.3%
Percentage methylation (CHG context) 18.3%
Percentage methylation (CHH context) 25.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 14372 original top strand
CTOT 18284 complementary to original top strand
CTOB 14371 complementary to original bottom strand
OB 10429 original bottom strand

Deduplication

Alignments analysed 57456
Unique alignments 24256
Duplicates removed 33200
Duplicated alignments were found at 4909 different positions

Cytosine Methylation after Extraction

Total C's analysed 181158
Methylated C's in CpG context 8337
Methylated C's in CHG context 3609
Methylated C's in CHH context 37785
Unmethylated C's in CpG context 10886
Unmethylated C's in CHG context 16185
Unmethylated C's in CHH context 104356
Percentage methylation (CpG context) 43.4%
Percentage methylation (CHG context) 18.2%
Percentage methylation (CHH context) 26.6%

M-Bias Plot