Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 56093
Sequences without alignments under any condition 59346303
Sequences that did not map uniquely 57035
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 504554
Methylated C's in CpG context 10126
Methylated C's in CHG context 6957
Methylated C's in CHH context 107706
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 17416
Unmethylated C's in CHG context 33622
Unmethylated C's in CHH context 328727
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 36.8%
Percentage methylation (CHG context) 17.1%
Percentage methylation (CHH context) 24.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 13926 original top strand
CTOT 18047 complementary to original top strand
CTOB 13674 complementary to original bottom strand
OB 10446 original bottom strand

Deduplication

Alignments analysed 56093
Unique alignments 24805
Duplicates removed 31288
Duplicated alignments were found at 4831 different positions

Cytosine Methylation after Extraction

Total C's analysed 184349
Methylated C's in CpG context 6654
Methylated C's in CHG context 3105
Methylated C's in CHH context 39493
Unmethylated C's in CpG context 11236
Unmethylated C's in CHG context 15761
Unmethylated C's in CHH context 108100
Percentage methylation (CpG context) 37.2%
Percentage methylation (CHG context) 16.5%
Percentage methylation (CHH context) 26.8%

M-Bias Plot