Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_12_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 59459431
Single-end alignments with a unique best hit 56093
Sequences without alignments under any condition 59346303
Sequences that did not map uniquely 57035
Genomic sequence context not extractable (edges of chromosomes) 0
0.0943%99.8%0.0959%0%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 504554
Methylated C's in CpG context 10126
Methylated C's in CHG context 6957
Methylated C's in CHH context 107706
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 17416
Unmethylated C's in CHG context 33622
Unmethylated C's in CHH context 328727
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 36.8%
Percentage methylation (CHG context) 17.1%
Percentage methylation (CHH context) 24.7%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 13926 original top strand
CTOT 18047 complementary to original top strand
CTOB 13674 complementary to original bottom strand
OB 10446 original bottom strand
OTCTOTCTOBOB02k4k6k8k10k12k14k16k18k
Number of Alignments

Deduplication

Alignments analysed 56093
Unique alignments 24805
Duplicates removed 31288
Duplicated alignments were found at 4831 different positions
44.2%55.8%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 184349
Methylated C's in CpG context 6654
Methylated C's in CHG context 3105
Methylated C's in CHH context 39493
Unmethylated C's in CpG context 11236
Unmethylated C's in CHG context 15761
Unmethylated C's in CHH context 108100
Percentage methylation (CpG context) 37.2%
Percentage methylation (CHG context) 16.5%
Percentage methylation (CHH context) 26.8%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

510152025300204060801000100020003000400050006000
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls