Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_11_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 54458572
Single-end alignments with a unique best hit 45087
Sequences without alignments under any condition 54368235
Sequences that did not map uniquely 45250
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 404025
Methylated C's in CpG context 12838
Methylated C's in CHG context 6669
Methylated C's in CHH context 86039
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 14080
Unmethylated C's in CHG context 28775
Unmethylated C's in CHH context 255624
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 47.7%
Percentage methylation (CHG context) 18.8%
Percentage methylation (CHH context) 25.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 11263 original top strand
CTOT 14371 complementary to original top strand
CTOB 11250 complementary to original bottom strand
OB 8203 original bottom strand

Deduplication

Alignments analysed 45087
Unique alignments 20372
Duplicates removed 24715
Duplicated alignments were found at 3893 different positions

Cytosine Methylation after Extraction

Total C's analysed 152158
Methylated C's in CpG context 7622
Methylated C's in CHG context 3046
Methylated C's in CHH context 30689
Unmethylated C's in CpG context 9109
Unmethylated C's in CHG context 13842
Unmethylated C's in CHH context 87850
Percentage methylation (CpG context) 45.6%
Percentage methylation (CHG context) 18.0%
Percentage methylation (CHH context) 25.9%

M-Bias Plot