Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/decomp//zr1394_10_s456_trimmed.fq

Data processed at 18:20 on 2022-06-16


Alignment Stats

Sequences analysed in total 78221457
Single-end alignments with a unique best hit 66725
Sequences without alignments under any condition 78101182
Sequences that did not map uniquely 53550
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 569706
Methylated C's in CpG context 17402
Methylated C's in CHG context 9468
Methylated C's in CHH context 126437
Methylated C's in Unknown context 0
Unmethylated C's in CpG context 20804
Unmethylated C's in CHG context 41768
Unmethylated C's in CHH context 353827
Unmethylated C's in Unknown context 0
Percentage methylation (CpG context) 45.5%
Percentage methylation (CHG context) 18.5%
Percentage methylation (CHH context) 26.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15820 original top strand
CTOT 20927 complementary to original top strand
CTOB 17831 complementary to original bottom strand
OB 12147 original bottom strand

Deduplication

Alignments analysed 66725
Unique alignments 25379
Duplicates removed 41346
Duplicated alignments were found at 7140 different positions

Cytosine Methylation after Extraction

Total C's analysed 183387
Methylated C's in CpG context 8263
Methylated C's in CHG context 3657
Methylated C's in CHH context 39733
Unmethylated C's in CpG context 11396
Unmethylated C's in CHG context 16606
Unmethylated C's in CHH context 103732
Percentage methylation (CpG context) 42.0%
Percentage methylation (CHG context) 18.0%
Percentage methylation (CHH context) 27.7%

M-Bias Plot