Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/7_CAGATC_L001_R1_001_trimmed.fq.gz

Data processed at 10:55:56 on 2021-06-14


Alignment Stats

Sequences analysed in total 10113854
Single-end alignments with a unique best hit 4732904
Sequences without alignments under any condition 144592
Sequences that did not map uniquely 5236358
Genomic sequence context not extractable (edges of chromosomes) 3226

Cytosine Methylation

Total C's analysed 26664014
Methylated C's in CpG context 903842
Methylated C's in CHG context 107795
Methylated C's in CHH context 2867247
Methylated C's in Unknown context 50394
Unmethylated C's in CpG context 2225682
Unmethylated C's in CHG context 4168082
Unmethylated C's in CHH context 16391366
Unmethylated C's in Unknown context 86742
Percentage methylation (CpG context) 28.9%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 14.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 1852528 original top strand
CTOT 513555 complementary to original top strand
CTOB 515474 complementary to original bottom strand
OB 1848121 original bottom strand

Deduplication

Alignments analysed 4729678
Unique alignments 4214077
Duplicates removed 515601
Duplicated alignments were found at 328557 different positions

Cytosine Methylation after Extraction

Total C's analysed 23724495
Methylated C's in CpG context 785155
Methylated C's in CHG context 93231
Methylated C's in CHH context 2435091
Unmethylated C's in CpG context 1991364
Unmethylated C's in CHG context 3690612
Unmethylated C's in CHH context 14729042
Percentage methylation (CpG context) 28.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot