Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/3_TTAGGC_L001_R1_001_trimmed.fq.gz (version: v0.22.3) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 11555321 Number of alignments with a unique best hit from the different alignments: 5436861 Mapping efficiency: 47.1% Sequences with no alignments under any condition: 173630 Sequences did not map uniquely: 5944830 Sequences which were discarded because genomic sequence could not be extracted: 3707 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 2126775 ((converted) top strand) CT/GA: 2119608 ((converted) bottom strand) GA/CT: 592511 (complementary to (converted) top strand) GA/GA: 594260 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 30884657 Total methylated C's in CpG context: 1070977 Total methylated C's in CHG context: 141123 Total methylated C's in CHH context: 3362649 Total methylated C's in Unknown context: 59075 Total unmethylated C's in CpG context: 2690299 Total unmethylated C's in CHG context: 4839624 Total unmethylated C's in CHH context: 18779985 Total unmethylated C's in Unknown context: 102723 C methylated in CpG context: 28.5% C methylated in CHG context: 2.8% C methylated in CHH context: 15.2% C methylated in Unknown context (CN or CHN): 36.5% Bismark completed in 0d 0h 23m 2s