Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/2_CGATGT_L001_R1_001_trimmed.fq.gz

Data processed at 10:55:55 on 2021-06-14


Alignment Stats

Sequences analysed in total 11654467
Single-end alignments with a unique best hit 5433833
Sequences without alignments under any condition 191064
Sequences that did not map uniquely 6029570
Genomic sequence context not extractable (edges of chromosomes) 3794

Cytosine Methylation

Total C's analysed 30793506
Methylated C's in CpG context 1054675
Methylated C's in CHG context 176932
Methylated C's in CHH context 3415378
Methylated C's in Unknown context 57018
Unmethylated C's in CpG context 2614904
Unmethylated C's in CHG context 4783710
Unmethylated C's in CHH context 18747907
Unmethylated C's in Unknown context 99509
Percentage methylation (CpG context) 28.7%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 15.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 2119792 original top strand
CTOT 596380 complementary to original top strand
CTOB 598303 complementary to original bottom strand
OB 2115564 original bottom strand

Deduplication

Alignments analysed 5430039
Unique alignments 4722183
Duplicates removed 707856
Duplicated alignments were found at 427792 different positions

Cytosine Methylation after Extraction

Total C's analysed 26662705
Methylated C's in CpG context 901196
Methylated C's in CHG context 150502
Methylated C's in CHH context 2806928
Unmethylated C's in CpG context 2236119
Unmethylated C's in CHG context 4126765
Unmethylated C's in CHH context 16441195
Percentage methylation (CpG context) 28.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot