Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/combined.fq.gz

Data processed at 12:31:50 on 2021-06-13


Alignment Stats

Sequences analysed in total 92334932
Single-end alignments with a unique best hit 40417426
Sequences without alignments under any condition 11929375
Sequences that did not map uniquely 39988131
Genomic sequence context not extractable (edges of chromosomes) 37499

Cytosine Methylation

Total C's analysed 217529869
Methylated C's in CpG context 8289193
Methylated C's in CHG context 521677
Methylated C's in CHH context 2297463
Methylated C's in Unknown context 31624
Unmethylated C's in CpG context 24139397
Unmethylated C's in CHG context 42918302
Unmethylated C's in CHH context 139363837
Unmethylated C's in Unknown context 541613
Percentage methylation (CpG context) 25.6%
Percentage methylation (CHG context) 1.2%
Percentage methylation (CHH context) 1.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 20219240 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 20160687 original bottom strand

Deduplication

Alignments analysed 40379927
Unique alignments 28573677
Duplicates removed 11806250
Duplicated alignments were found at 5277569 different positions

Cytosine Methylation after Extraction

Total C's analysed 154138678
Methylated C's in CpG context 5284600
Methylated C's in CHG context 343750
Methylated C's in CHH context 1627489
Unmethylated C's in CpG context 16537973
Unmethylated C's in CHG context 29141338
Unmethylated C's in CHH context 101203528
Percentage methylation (CpG context) 24.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot