Bismark report for: /gscratch/srlab/sr320/data/olurida-bs/combined.fq.gz (version: v0.22.3) Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/olurida-bs/genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals Final Alignment report ====================== Sequences analysed in total: 92334932 Number of alignments with a unique best hit from the different alignments: 43226506 Mapping efficiency: 46.8% Sequences with no alignments under any condition: 1408355 Sequences did not map uniquely: 47700071 Sequences which were discarded because genomic sequence could not be extracted: 29930 Number of sequences with unique best (first) alignment came from the bowtie output: CT/CT: 16867827 ((converted) top strand) CT/GA: 16848105 ((converted) bottom strand) GA/CT: 4733711 (complementary to (converted) top strand) GA/GA: 4746933 (complementary to (converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 244706621 Total methylated C's in CpG context: 8302900 Total methylated C's in CHG context: 1148320 Total methylated C's in CHH context: 26772276 Total methylated C's in Unknown context: 465478 Total unmethylated C's in CpG context: 20955820 Total unmethylated C's in CHG context: 38244673 Total unmethylated C's in CHH context: 149282632 Total unmethylated C's in Unknown context: 808211 C methylated in CpG context: 28.4% C methylated in CHG context: 2.9% C methylated in CHH context: 15.2% C methylated in Unknown context (CN or CHN): 36.5% Bismark completed in 0d 3h 0m 56s