Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/olurida-bs/combined.fq.gz

Data processed at 11:10:46 on 2021-06-11


Alignment Stats

Sequences analysed in total 92334932
Single-end alignments with a unique best hit 43226506
Sequences without alignments under any condition 1408355
Sequences that did not map uniquely 47700071
Genomic sequence context not extractable (edges of chromosomes) 29930
46.8%1.52%51.6%0.0324%
Unique AlignmentsNo AlignmentMultiple AlignmentsNo Genomic Sequence

Cytosine Methylation

Total C's analysed 244706621
Methylated C's in CpG context 8302900
Methylated C's in CHG context 1148320
Methylated C's in CHH context 26772276
Methylated C's in Unknown context 465478
Unmethylated C's in CpG context 20955820
Unmethylated C's in CHG context 38244673
Unmethylated C's in CHH context 149282632
Unmethylated C's in Unknown context 808211
Percentage methylation (CpG context) 28.4%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 15.2%
Methylated C's in Unknown context N/A%
CpG contextCHG contextCHH context020406080100
% Methylation

Alignment to Individual Bisulfite Strands

OT 16867827 original top strand
CTOT 4733711 complementary to original top strand
CTOB 4746933 complementary to original bottom strand
OB 16848105 original bottom strand
OTCTOTCTOBOB02M4M6M8M10M12M14M16M
Number of Alignments

Deduplication

Alignments analysed 43196576
Unique alignments 30566851
Duplicates removed 12629725
Duplicated alignments were found at 5862400 different positions
70.8%29.2%
Unique AlignmentsDuplicate Alignments

Cytosine Methylation after Extraction

Total C's analysed 171722801
Methylated C's in CpG context 5436165
Methylated C's in CHG context 783128
Methylated C's in CHH context 16578021
Unmethylated C's in CpG context 14720586
Unmethylated C's in CHG context 26603527
Unmethylated C's in CHH context 107601374
Percentage methylation (CpG context) 27.0%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 13.4%
CpG contextCHH contextCHG context020406080100
% Methylation

M-Bias Plot

510152025300204060801000.5M1M1.5M2M2.5M3M3.5M4M
CpG methylationCHG methylationCHH methylationCpG total callsCHG total callsCHH total callsRead 1Position in Read [bp]% Methylation# Methylation Calls