Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_9_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_9_R2.fq.gz

Data processed at 23:10:15 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60757
Pairs without alignments under any condition 21589
Pairs that did not map uniquely 17654
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2120011
Methylated C's in CpG context 30085
Methylated C's in CHG context 1759
Methylated C's in CHH context 7647
Methylated C's in Unknown context 80
Unmethylated C's in CpG context 246978
Unmethylated C's in CHG context 370481
Unmethylated C's in CHH context 1463061
Unmethylated C's in Unknown context 4956
Percentage methylation (CpG context) 10.9%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15319 original top strand
CTOT 14927 complementary to original top strand
CTOB 15269 complementary to original bottom strand
OB 15242 original bottom strand

Deduplication

Alignments analysed 60757
Unique alignments 56393
Duplicates removed 4364
Duplicated alignments were found at 4061 different positions

Cytosine Methylation after Extraction

Total C's analysed 1289022
Methylated C's in CpG context 17731
Methylated C's in CHG context 1087
Methylated C's in CHH context 4608
Unmethylated C's in CpG context 148754
Unmethylated C's in CHG context 223139
Unmethylated C's in CHH context 893703
Percentage methylation (CpG context) 10.7%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot