Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
60858 |
| Pairs without alignments under any condition |
22549 |
| Pairs that did not map uniquely |
16593 |
| Genomic sequence context not extractable (edges of chromosomes) |
1 |
|
|
Cytosine Methylation
| Total C's analysed |
2144468 |
| Methylated C's in CpG context |
29213 |
| Methylated C's in CHG context |
1771 |
| Methylated C's in CHH context |
7827 |
| Methylated C's in Unknown context |
73 |
| Unmethylated C's in CpG context |
248391 |
| Unmethylated C's in CHG context |
372784 |
| Unmethylated C's in CHH context |
1484482 |
| Unmethylated C's in Unknown context |
5270 |
| Percentage methylation (CpG context) |
10.5% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15316 |
original top strand |
| CTOT |
15202 |
complementary to original top strand |
| CTOB |
15237 |
complementary to original bottom strand |
| OB |
15102 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
60857 |
| Unique alignments |
57165 |
| Duplicates removed |
3692 |
| Duplicated alignments were found at 3470 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1375616 |
| Methylated C's in CpG context |
18119 |
| Methylated C's in CHG context |
1126 |
| Methylated C's in CHH context |
5002 |
| Unmethylated C's in CpG context |
158953 |
| Unmethylated C's in CHG context |
237093 |
| Unmethylated C's in CHH context |
955323 |
| Percentage methylation (CpG context) |
10.2% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot