Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_8_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_8_R2.fq.gz

Data processed at 23:10:15 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60858
Pairs without alignments under any condition 22549
Pairs that did not map uniquely 16593
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 2144468
Methylated C's in CpG context 29213
Methylated C's in CHG context 1771
Methylated C's in CHH context 7827
Methylated C's in Unknown context 73
Unmethylated C's in CpG context 248391
Unmethylated C's in CHG context 372784
Unmethylated C's in CHH context 1484482
Unmethylated C's in Unknown context 5270
Percentage methylation (CpG context) 10.5%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15316 original top strand
CTOT 15202 complementary to original top strand
CTOB 15237 complementary to original bottom strand
OB 15102 original bottom strand

Deduplication

Alignments analysed 60857
Unique alignments 57165
Duplicates removed 3692
Duplicated alignments were found at 3470 different positions

Cytosine Methylation after Extraction

Total C's analysed 1375616
Methylated C's in CpG context 18119
Methylated C's in CHG context 1126
Methylated C's in CHH context 5002
Unmethylated C's in CpG context 158953
Unmethylated C's in CHG context 237093
Unmethylated C's in CHH context 955323
Percentage methylation (CpG context) 10.2%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot