Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_7_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_7_R2.fq.gz

Data processed at 23:10:15 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60320
Pairs without alignments under any condition 23556
Pairs that did not map uniquely 16124
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2133451
Methylated C's in CpG context 28548
Methylated C's in CHG context 1785
Methylated C's in CHH context 7249
Methylated C's in Unknown context 57
Unmethylated C's in CpG context 245609
Unmethylated C's in CHG context 367100
Unmethylated C's in CHH context 1483160
Unmethylated C's in Unknown context 5320
Percentage methylation (CpG context) 10.4%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15104 original top strand
CTOT 14956 complementary to original top strand
CTOB 15142 complementary to original bottom strand
OB 15118 original bottom strand

Deduplication

Alignments analysed 60320
Unique alignments 56845
Duplicates removed 3475
Duplicated alignments were found at 3290 different positions

Cytosine Methylation after Extraction

Total C's analysed 1385784
Methylated C's in CpG context 18157
Methylated C's in CHG context 1148
Methylated C's in CHH context 4695
Unmethylated C's in CpG context 158602
Unmethylated C's in CHG context 236768
Unmethylated C's in CHH context 966414
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot