Alignment Stats
Sequence pairs analysed in total |
100000 |
Paired-end alignments with a unique best hit |
61547 |
Pairs without alignments under any condition |
22163 |
Pairs that did not map uniquely |
16290 |
Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
Total C's analysed |
2173592 |
Methylated C's in CpG context |
28924 |
Methylated C's in CHG context |
1803 |
Methylated C's in CHH context |
7453 |
Methylated C's in Unknown context |
55 |
Unmethylated C's in CpG context |
250847 |
Unmethylated C's in CHG context |
374871 |
Unmethylated C's in CHH context |
1509694 |
Unmethylated C's in Unknown context |
5258 |
Percentage methylation (CpG context) |
10.3% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
OT |
15381 |
original top strand |
CTOT |
15556 |
complementary to original top strand |
CTOB |
15289 |
complementary to original bottom strand |
OB |
15321 |
original bottom strand |
|
|
Deduplication
Alignments analysed |
61547 |
Unique alignments |
57662 |
Duplicates removed |
3885 |
Duplicated alignments were found at 3613 different positions |
|
|
Cytosine Methylation after Extraction
Total C's analysed |
1394445 |
Methylated C's in CpG context |
18046 |
Methylated C's in CHG context |
1139 |
Methylated C's in CHH context |
4804 |
Unmethylated C's in CpG context |
160195 |
Unmethylated C's in CHG context |
238321 |
Unmethylated C's in CHH context |
971940 |
Percentage methylation (CpG context) |
10.1% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot