Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
61547 |
| Pairs without alignments under any condition |
22163 |
| Pairs that did not map uniquely |
16290 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2173592 |
| Methylated C's in CpG context |
28924 |
| Methylated C's in CHG context |
1803 |
| Methylated C's in CHH context |
7453 |
| Methylated C's in Unknown context |
55 |
| Unmethylated C's in CpG context |
250847 |
| Unmethylated C's in CHG context |
374871 |
| Unmethylated C's in CHH context |
1509694 |
| Unmethylated C's in Unknown context |
5258 |
| Percentage methylation (CpG context) |
10.3% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15381 |
original top strand |
| CTOT |
15556 |
complementary to original top strand |
| CTOB |
15289 |
complementary to original bottom strand |
| OB |
15321 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
61547 |
| Unique alignments |
57662 |
| Duplicates removed |
3885 |
| Duplicated alignments were found at 3613 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1394445 |
| Methylated C's in CpG context |
18046 |
| Methylated C's in CHG context |
1139 |
| Methylated C's in CHH context |
4804 |
| Unmethylated C's in CpG context |
160195 |
| Unmethylated C's in CHG context |
238321 |
| Unmethylated C's in CHH context |
971940 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot