Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_6_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_6_R2.fq.gz

Data processed at 23:10:15 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61547
Pairs without alignments under any condition 22163
Pairs that did not map uniquely 16290
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2173592
Methylated C's in CpG context 28924
Methylated C's in CHG context 1803
Methylated C's in CHH context 7453
Methylated C's in Unknown context 55
Unmethylated C's in CpG context 250847
Unmethylated C's in CHG context 374871
Unmethylated C's in CHH context 1509694
Unmethylated C's in Unknown context 5258
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15381 original top strand
CTOT 15556 complementary to original top strand
CTOB 15289 complementary to original bottom strand
OB 15321 original bottom strand

Deduplication

Alignments analysed 61547
Unique alignments 57662
Duplicates removed 3885
Duplicated alignments were found at 3613 different positions

Cytosine Methylation after Extraction

Total C's analysed 1394445
Methylated C's in CpG context 18046
Methylated C's in CHG context 1139
Methylated C's in CHH context 4804
Unmethylated C's in CpG context 160195
Unmethylated C's in CHG context 238321
Unmethylated C's in CHH context 971940
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot