Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_4_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_4_R2.fq.gz

Data processed at 23:10:14 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 61001
Pairs without alignments under any condition 22625
Pairs that did not map uniquely 16374
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2155823
Methylated C's in CpG context 28443
Methylated C's in CHG context 1706
Methylated C's in CHH context 6933
Methylated C's in Unknown context 89
Unmethylated C's in CpG context 247462
Unmethylated C's in CHG context 369756
Unmethylated C's in CHH context 1501523
Unmethylated C's in Unknown context 5203
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15211 original top strand
CTOT 15263 complementary to original top strand
CTOB 15204 complementary to original bottom strand
OB 15323 original bottom strand

Deduplication

Alignments analysed 61001
Unique alignments 56883
Duplicates removed 4118
Duplicated alignments were found at 3871 different positions

Cytosine Methylation after Extraction

Total C's analysed 1373862
Methylated C's in CpG context 17554
Methylated C's in CHG context 1064
Methylated C's in CHH context 4411
Unmethylated C's in CpG context 156232
Unmethylated C's in CHG context 233363
Unmethylated C's in CHH context 961238
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot