Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_3_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_3_R2.fq.gz

Data processed at 23:10:14 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60870
Pairs without alignments under any condition 22616
Pairs that did not map uniquely 16514
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2151592
Methylated C's in CpG context 28625
Methylated C's in CHG context 1778
Methylated C's in CHH context 7188
Methylated C's in Unknown context 63
Unmethylated C's in CpG context 250192
Unmethylated C's in CHG context 372662
Unmethylated C's in CHH context 1491147
Unmethylated C's in Unknown context 5347
Percentage methylation (CpG context) 10.3%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15284 original top strand
CTOT 15322 complementary to original top strand
CTOB 15195 complementary to original bottom strand
OB 15069 original bottom strand

Deduplication

Alignments analysed 60870
Unique alignments 57222
Duplicates removed 3648
Duplicated alignments were found at 3414 different positions

Cytosine Methylation after Extraction

Total C's analysed 1388599
Methylated C's in CpG context 17982
Methylated C's in CHG context 1129
Methylated C's in CHH context 4596
Unmethylated C's in CpG context 160404
Unmethylated C's in CHG context 238481
Unmethylated C's in CHH context 966007
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot