Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_2_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_2_R2.fq.gz

Data processed at 23:10:14 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 60791
Pairs without alignments under any condition 23155
Pairs that did not map uniquely 16054
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2172850
Methylated C's in CpG context 28420
Methylated C's in CHG context 1893
Methylated C's in CHH context 7850
Methylated C's in Unknown context 81
Unmethylated C's in CpG context 252139
Unmethylated C's in CHG context 374344
Unmethylated C's in CHH context 1508204
Unmethylated C's in Unknown context 5353
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15044 original top strand
CTOT 14926 complementary to original top strand
CTOB 15404 complementary to original bottom strand
OB 15417 original bottom strand

Deduplication

Alignments analysed 60791
Unique alignments 56666
Duplicates removed 4125
Duplicated alignments were found at 3834 different positions

Cytosine Methylation after Extraction

Total C's analysed 1419476
Methylated C's in CpG context 18105
Methylated C's in CHG context 1208
Methylated C's in CHH context 5066
Unmethylated C's in CpG context 163850
Unmethylated C's in CHG context 242924
Unmethylated C's in CHH context 988323
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot