Alignment Stats
| Sequence pairs analysed in total |
100000 |
| Paired-end alignments with a unique best hit |
60791 |
| Pairs without alignments under any condition |
23155 |
| Pairs that did not map uniquely |
16054 |
| Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
| Total C's analysed |
2172850 |
| Methylated C's in CpG context |
28420 |
| Methylated C's in CHG context |
1893 |
| Methylated C's in CHH context |
7850 |
| Methylated C's in Unknown context |
81 |
| Unmethylated C's in CpG context |
252139 |
| Unmethylated C's in CHG context |
374344 |
| Unmethylated C's in CHH context |
1508204 |
| Unmethylated C's in Unknown context |
5353 |
| Percentage methylation (CpG context) |
10.1% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
| Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
| OT |
15044 |
original top strand |
| CTOT |
14926 |
complementary to original top strand |
| CTOB |
15404 |
complementary to original bottom strand |
| OB |
15417 |
original bottom strand |
|
|
Deduplication
| Alignments analysed |
60791 |
| Unique alignments |
56666 |
| Duplicates removed |
4125 |
| Duplicated alignments were found at 3834 different positions |
|
|
Cytosine Methylation after Extraction
| Total C's analysed |
1419476 |
| Methylated C's in CpG context |
18105 |
| Methylated C's in CHG context |
1208 |
| Methylated C's in CHH context |
5066 |
| Unmethylated C's in CpG context |
163850 |
| Unmethylated C's in CHG context |
242924 |
| Unmethylated C's in CHH context |
988323 |
| Percentage methylation (CpG context) |
10.0% |
| Percentage methylation (CHG context) |
0.5% |
| Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot