Alignment Stats
Sequence pairs analysed in total |
100000 |
Paired-end alignments with a unique best hit |
62084 |
Pairs without alignments under any condition |
21776 |
Pairs that did not map uniquely |
16140 |
Genomic sequence context not extractable (edges of chromosomes) |
0 |
|
|
Cytosine Methylation
Total C's analysed |
2208035 |
Methylated C's in CpG context |
29099 |
Methylated C's in CHG context |
1864 |
Methylated C's in CHH context |
7332 |
Methylated C's in Unknown context |
58 |
Unmethylated C's in CpG context |
258886 |
Unmethylated C's in CHG context |
384718 |
Unmethylated C's in CHH context |
1526136 |
Unmethylated C's in Unknown context |
5293 |
Percentage methylation (CpG context) |
10.1% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
Methylated C's in Unknown context |
N/A% |
|
|
Alignment to Individual Bisulfite Strands
OT |
15467 |
original top strand |
CTOT |
15609 |
complementary to original top strand |
CTOB |
15375 |
complementary to original bottom strand |
OB |
15633 |
original bottom strand |
|
|
Deduplication
Alignments analysed |
62084 |
Unique alignments |
57896 |
Duplicates removed |
4188 |
Duplicated alignments were found at 3945 different positions |
|
|
Cytosine Methylation after Extraction
Total C's analysed |
1408564 |
Methylated C's in CpG context |
18158 |
Methylated C's in CHG context |
1181 |
Methylated C's in CHH context |
4648 |
Unmethylated C's in CpG context |
164256 |
Unmethylated C's in CHG context |
243584 |
Unmethylated C's in CHH context |
976737 |
Percentage methylation (CpG context) |
10.0% |
Percentage methylation (CHG context) |
0.5% |
Percentage methylation (CHH context) |
0.5% |
|
|
M-Bias Plot