Bismark

Bismark Processing Report

/gscratch/srlab/sr320/data/cg-big/zrtg3644_24_R1.fq.gz and /gscratch/srlab/sr320/data/cg-big/zrtg3644_24_R2.fq.gz

Data processed at 23:10:14 on 2021-06-09


Alignment Stats

Sequence pairs analysed in total 100000
Paired-end alignments with a unique best hit 62084
Pairs without alignments under any condition 21776
Pairs that did not map uniquely 16140
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 2208035
Methylated C's in CpG context 29099
Methylated C's in CHG context 1864
Methylated C's in CHH context 7332
Methylated C's in Unknown context 58
Unmethylated C's in CpG context 258886
Unmethylated C's in CHG context 384718
Unmethylated C's in CHH context 1526136
Unmethylated C's in Unknown context 5293
Percentage methylation (CpG context) 10.1%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 15467 original top strand
CTOT 15609 complementary to original top strand
CTOB 15375 complementary to original bottom strand
OB 15633 original bottom strand

Deduplication

Alignments analysed 62084
Unique alignments 57896
Duplicates removed 4188
Duplicated alignments were found at 3945 different positions

Cytosine Methylation after Extraction

Total C's analysed 1408564
Methylated C's in CpG context 18158
Methylated C's in CHG context 1181
Methylated C's in CHH context 4648
Unmethylated C's in CpG context 164256
Unmethylated C's in CHG context 243584
Unmethylated C's in CHH context 976737
Percentage methylation (CpG context) 10.0%
Percentage methylation (CHG context) 0.5%
Percentage methylation (CHH context) 0.5%

M-Bias Plot